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]]>The present invention relates to a system and method for the manufacture of a biological tissue based product. More particularly, the present invention relates to a system and method for the manufacture of a biological tissue based product capable of being transformed into an artificial skin substitute or a blood vessel.
Tissue-engineered products are increasingly being used in the medical field to treat injuries to the skin or to repair damaged internal organs. In particular, various synthetic materials have been employed to replace or repair damaged tissue. Such materials include polytetrafluoroethylene (PTFE), polyurethane, silicone, and various semi-permeable polymeric materials. Nevertheless, such materials have serious drawbacks. For example, synthetic vascular grafts have a relatively high failure rate. Further, it is exceedingly difficult to achieve uniform, smooth, non-thrombogenic surfaces, and non-conductive material.
Another drawback of current synthetic vascular grafts is that they have a very limited lifetime. The synthetic grafts will atrophy, intimal hyperplasia will form, thrombi, and tissue necrosis will occur within a matter of months. They must, therefore, be replaced frequently, which is expensive and traumatic to the patient.
To solve the problems associated with synthetic grafts, many tissue engineering companies have manufactured a class of products known as “in-situ” vascular grafts. In-situ vascular grafts are typically constructed by seeding or coating appropriate components of a tube onto a stent. The components are typically collagen or collagen/glycosaminoglycan compositions, which provide mechanical and biological properties to the vascular grafts. It has been found that the in-situ products mimic the micro-anatomy of natural blood vessels in a manner that at least partially solves the problems associated with synthetic vascular grafts.
It has further been found that in-situ vascular grafts may be produced by first creating a tubular shaped stent. The stent is typically formed of a metal or metal alloy, such as stainless steel, or is a shape memory polymer. The stent has a plurality of preselected apertures therethrough. The stent is then loaded with a biocompatible solution of collagen or a collagen/glycosaminoglycan composition that is cross-linked to provide structural integrity to the collagen. For example, U.S. Pat. No. 5,782,903, the disclosure of which is incorporated by reference herein, discl