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Name	Hunt: Showdown – Live by the Blade
Publisher	giaduck
Format	File
Rating	4.70 / 5 ( 5197 votes )
Update	(6 days ago)

 

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Return to the swamps of Louisiana and embark on a ghostly journey into the unforgiving bayou in the first part of the long-awaited DLC, Farrier’s Fist!
Hunt alongside bandits and the undead as you explore nine new missions. Sharpen your bayou hunting prowess with the deadliest melee weapons available!
7 new weapons with a wide variety of skills and use for each.
More than 35 new animations!
3 new enemies!
New water effects!
18 new artifacts!
Immerse yourself in Louisiana in the New Orleans Bayou and co-op with your friends! *eLife* **10**:e06811.

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The discovery of massive parallel gRNA libraries was a major breakthrough in CRISPR/Cas9 editing. The widespread use of these libraries enabled a rapid pace of introducing mutations into mammalian genomes. Nonetheless, they are not perfect. As shown in [@bib1], one of the major issues for *in vitro* cell-based systems was that library bias could influence the resulting mutagenic spectrum. Although the accuracy of using *in vitro* gRNA libraries for genome editing could be promising in some cases, the robustness remains to be established.

In a recent paper published in *eLife*, [@bib2] present data suggesting a bottleneck in the CRISPR/Cas9 genome editing process. They report that the use of *in vitro*-selected gRNAs did not lead to the expected increases in the incidence of mutations in targeted regions of the mammalian genome. Instead, they show that the selected gRNAs generate a mutagenic spectrum biased to repeat sequences, such as transposons. This creates concerns about the robustness and reproducibility of the use of *in vitro*-selected libraries for genome editing.

In the study of [@bib2], very few gRNAs were tested *in vivo*. Therefore, the library source is not a concern as long as every single gRNA was selected from a population. On the other hand, the gRNAs tested *in vitro* were introduced in an extrachromosomal plasmid, which could lead to false-positive results.

A major issue in the study of [@bib2] is that, at least to our knowledge, they used 5′-phosphate-dependent crRNAs. These crRNAs have a heterogenous

 

Hunt: Showdown – Live By The Blade Features Key:

• Catch more than 30 completely unique online beasts
• Hunt undead-like monsters that completely defy logic
• Overcome more than 30 unique hazards
• Fight mob after mob with electrified claws, fire, webs, and shurikens
• Face off against online opponents that blend together to create unique experiences
• Play through countless challenges to find the best gear for your style
• Unlock epic endings and create an auto-save record
• Unlock new gear and recipes and level up an unlimited amount of abilities

Key Features:

• Ready to play right away. No need to wait for patches or updates.
• Lots of unique content and challenges in 3 stunning environments.
• Create an auto save.
• Xbox LIVE Leaderboards for all game modes.