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Enhanced permeability of gold nanoparticles (AuNPs) through biological barriers will allow them to penetrate into the sites of injury. Herein, a recombinant form of superoxide dismutase 3 (SOD3) was cloned into pET-30a and was expressed in Escherichia coli BL21 (DE3). AuNP-SOD3 was then purified using anion-exchange chromatography. The solution UV-vis spectra indicated a bathochromic shift (∼30 nm), a red-shift (∼150 nm) and a decreased absorbance at 450 nm (∼40%) as a result of the new band centered at 595 nm. The zeta potential was ∼21 mV, and the diameter was 46.4 ± 3.7 nm. The TEM micrographs showed spherical shape of AuNP-SOD3 with the homogeneous size distribution. AuNPs-SOD3 were tested using the traditional method, while the effect on SOD3 activity was evaluated using the standard reaction of xanthine oxidation and the ABTS˙+ method. AuNPs-SOD3, but not AuNPs, effectively enhanced the SOD3 activity by 30%. Meanwhile, the AuNPs-SOD3 mediated hydroxyl radical inhibition ability was enhanced by 30% and 50%. In addition, AuNPs-SOD3 was found to translocate from the surface of E. coli to the inside of the cell (∼0.4 cm inside of bacterial cell wall).![](edinbmedj74506-0056){#sp1.238}
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